H3K9 dimethyltransferase G9a is an important epigenetic modulator of B cell differentiation

Abstract Plasma cell differentiation is a tightly regulated process coordinated by the timed expression of several transcription factors as well histone modifying enzymes that modulate chromatin accessibility. During this process, methyltransferase (HMT) G9a, dimethylates H3 lysine 9 (H3K9) at promoters and inhibits gene through recruitment proteins impair HMTs are expressed ubiquitously but display distinct enzymatic activities patterns chromosomal localization. plasma differentiation, G9a was found to co-localize with Blimp-1, which required silence genes associated B fate cellular proliferation. However, processes modulated mediated dimethylation during formation remain be elucidated. To study role in we crossed fl/flmice onto CD19 Cre/+background (G9aKO mice). Stimulation Cre/+(CreCtrl) G9aKO mice T independent antigen LPS resulted significant increase activated cells plasmablast mice. Further characterization phenotype, identified skewing mature sub-populations mice, accompanied altered proliferation rates when challenged. ATAC-Seq RNA-Seq will used identify accessibility changes deficient The CUT&Tag assay also validate regions subject direct modulation differentiation. Together, our data suggests contributes regulating his work supported grants from NIH/NIAID (RO1 AI123733 P01 AI125180 JMB)